Increased sister chromatid exchange in peripheral blood lymphocytes from humans exposed to pesticide: evidence based on a meta-analysis.

Hai-Yan Yang,Si-Yu Yang,Jing Liu,Ya-Dong Wang,Hai-Yu Wang

doi:10.7314/apjcp.2014.15.22.9725

Hai-Yan Yang, Si-Yu Yang + Show 3 more

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https://doi.org/10.7314/apjcp.2014.15.22.9725

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Abstract

Sister chromatid exchange (SCE) in human peripheral blood lymphocytes is one of the most extensively studied biomarkers employed to evaluate genetic damage subsequent to pesticide exposure. To estimate the pooled levels of SCE in human peripheral blood lymphocytes among population exposed to pesticide. Meta-analysis on the association between SCE frequency and pesticide exposure was performed with STATA 10.0 software package and Review Manager 5.0.24 in this study. The overall means of SCE were 7.88 [95% confidence intervals (95%CI): 6.71-9.04] for exposure group and 6.05 (95%CI: 5.13-6.95) for controls, respectively. There was statistically significant difference in the SCE frequency in human peripheral blood lymphocytes between pesticide-exposed groups and control groups, and the summary estimate of weighted mean difference was 1.69 (95%CI: 1.01-2.38). We also observed that pesticide-exposed population had significantly higher SCE frequency than control groups among smokers, nonsmokers, pesticide applicator, pesticide producer, other exposure population and Asian population in stratified analyses. Data indicate that the SCE frequency in human peripheral blood lymphocytes might be an indicator of early genetic effects for pesticide-exposed populations.

Highlights

Pesticides are a group of natural or synthetic chemical substances including insecticide, herbicide and fungicide, being designated to combat plagues that generally attack, harm or transmit illness to living organisms including humans
Sister chromatid exchange (SCE) results from S-dependent lesions which during S-phase are eventually transformed to double strand break (DSB) and these can be repaired by a homologous recombination mechanism that may give rise to SCE
Nikoloff et al reported that herbicide flurochloridone induced a significant and equivalent increase in SCEs regardless of the concentration in Chinese hamster ovary cells treated for 24h within the 0.25-15μg/ml concentration range (Nikoloff et al, 2012)

Summary

Introduction

Pesticides are a group of natural or synthetic chemical substances including insecticide, herbicide and fungicide, being designated to combat plagues that generally attack, harm or transmit illness to living organisms including humans. Sister chromatid exchange (SCE) in human peripheral blood lymphocytes is one of the most extensively studied biomarkers of cytogenetic damage. Sister chromatid exchange (SCE) in human peripheral blood lymphocytes is one of the most extensively studied biomarkers employed to evaluate genetic damage subsequent to pesticide exposure. Objective: To estimate the pooled levels of SCE in human peripheral blood lymphocytes among population exposed to pesticide. There was statistically significant difference in the SCE frequency in human peripheral blood lymphocytes between pesticide-exposed groups and control groups, and the summary estimate of weighted mean difference was 1.69 (95%CI: 1.01-2.38). Conclusions: Data indicate that the SCE frequency in human peripheral blood lymphocytes might be an indicator of early genetic esffects for pesticide-exposed populations.

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