Abstract
β-Cyclodextrin(β-CD) was produced by cyclodextrin glycosyltransferase(CGTase) in aqueous two-phase system. β-CD production from soluble starch was catalyzed by CGTase in dextran-rich bottom phase, and the β-CD produced was transferred to PEG-rich top phase in aqueous two-phase system, composed of 7% (w/w) polyethylene glycol(Mr 20,000) and 10% (w/w) dextran(Mr 38,900). Partition coefficients of β-CD and CGTase were 1.5 and 0.25, respectively. The total productivity of β-CD in aqueous two-phase system was about 3 times of that in dextran phase.
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