INCREASED PLASMA IL‐1 CONCENTRATIONS IN POLYCYSTIC OVARYINDUCED RATS

Abstract

Polycystic ovary syndrome (PCOS) is a clinical disorder that affects up to 15% of women of reproductive age, and is the most common endocrine disorder among pre‐menopausal women. PCOS is characterized by impairment of ovulation, reduced fertility, and imbalance of reproductive hormones. In addition, women with PCOS often exhibit obesity, metabolic syndrome, hyperinsulinemia, insulin resistance, and dyslipidemia. Adipose tissue secretes hormones that contribute to processes that regulate metabolism, inflammatory responses, immunity, and reproduction. In obesity, body fat deposits are hypertrophied, stimulated by androgens that influence the expression of enzymes and proteins involved in lipid and carbohydrate metabolism, oxidative stress, and differentiation of pre‐adipocytes into mature adipocytes. A consequence of hypertrophy and adipose hyperplasia is the expression of pro‐inflammatory cytokines. The goal of this study was to analyze the plasma variations of interleukin‐1 (IL‐1) in rats induced to polycystic ovary (PCO). Ten female adult Wistar rats weighing 200–340 g were divided into control (n = 5) and PCO (n = 5) groups. PCO was induced in the rats by single injection of estradiol valerate (EV) (2 mg/kg/bw, i.m.). The two groups of rats were then evaluated after 30 days. The results were reported as means ± SEM. Statistically significant differences among the means for the treatment groups were identified using p<0.05. This study was approved by the local Animal Care and Use Committee (CEUA–UNIARA, protocol n° 031/16). The results showed that the body mass of the control group (344 ± 13.56 g) was higher than that of the PCO group (306.4 ± 32.35 g). The ovary mass of the control group (0.072 ± 0.004 g) was higher than for the PCO group (0.048 ± 0.005 g), and the gonadal somatic index of the control group (0.0257 ± 0.006) was also higher than for the PCO group (0.0166 ± 0.008). Finally, the plasma IL‐1 concentration of the PCO group (55.68 ± 13.15 pg/mL) was higher than for the control group (21.62 ± 5.78 pg/mL). The results were indicative of metabolic and inflammatory changes in the rats 30 days after the PCO induction.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.