Abstract
A quantitative cytochemical study of glucose-6-phosphate dehydrogenase activity as a marker of the pentose phosphate pathway (PPP) was made on shoot apices of Spinacia oleracea kept either continuously in short days for up to eight weeks or transferred for a single period of 20 h to continuous light after between three and eight weeks in short days. By the time the apices kept in continuous short days showed morphological changes relating to the floral state, the PPP activity was already elevated. From four weeks onwards, the apices were more readily induced to the floral state as evidenced by the increased PPP activity. In addition, the level of PPP activity achieved in the short-day apices as they progressed to the floral state was as great as that observed in the apices induced to flower by a 20-h day.
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