Abstract
SPECIFIC AIMSWe tested the hypothesis that increased ribosome biogenesis is a prerequisite for the progression of cardiomyocyte hypertrophic growth and that this process is regulated at the level of ribosomal gene (rDNA) transcription by the nucleolar transcription factor UBF.PRINCIPAL FINDINGS1. Adenovirus-directed expression of UBF antisense RNA inhibits α1-adrenergic- and contraction-induced UBF expression in cardiomyocytesNoncontracting primary cultures of neonatal cardiac muscle cells (cardiomyocytes) were infected at a titer of 1 × 102 PFU/cell with either a control Ad-5-derived virus expressing the nonmammalian, control gene chloramphenicol transferase (Ad-CAT) or an Ad-5 virus expressing UBF cDNA (−267 to+635) in the antisense orientation under control of the CMV promoter (Ad-UBFas). At this titer, 95% of the myocytes were infected as determined by GFP fluorescence. After infection, the cardiomyocytes were stimulated for 3 days with either the α1-adrenergic agonist phenylephrine (PE; 25 μM) or con...
Published Version
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