Increased chemerin expression in the peritoneal fluid and ectopic endometrium of women with endometriosis

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ObjectiveInflammation is an essential process in the development and growth of endometriosis. Chemerin is a recently found adipokine that modulates chemotaxis and activation of macrophages. Previous studies have shown that chemerin have both pro- and anti-inflammatory properties. Given the evidence that various inflammatory cytokines and chemokines are aberrant in endometriosis, chemerin may have biological relevance for the pathogenesis of endometriosis.DesignThis study was to examine the chemerin levels in serum and peritoneal fluid (PF) and chemerin mRNA expression from the ectopic and eutopic endometrium of women with or without endometriosis.Materials and methodsThe serum and PF samples were obtained from eighty women (30 women with endometriosis and 41 controls). The concentrations of chemerin in serum and PF from the subjects were determined by the enzyme-linked immunosorbent assay. The mRNA expression of chemerin in the ectopic and eutopic endometrium was measured using with real time RT-PCR.ResultsThe mean concentrations of PF chemerin were significantly higher in women with endometriosis (4.17±0.1ng/mL) compared than those of the controls (3.5±0.1 ng/mL) (P<0.001). The serum levels of chemerin did not differ between the two groups. In analysis of real time RT-PCR with the ectopic endometrial tissue from the ovary (N=20) and normal eutopic endometrium of the controls (N=20), the chemerin mRNA expression was significantly higher in the ectopic endometrium compared than that in the eutopic endometrium of women without endometriosis (P=0.004).ConclusionWe observed that chemerin, a potential inflammatory mediator, significantly increased in endometriotic PF and was highly expressed from the ectopic endometrial cells. The results of this study suggest that chemerin may play an important role in the pathogenesis of endometriosis. ObjectiveInflammation is an essential process in the development and growth of endometriosis. Chemerin is a recently found adipokine that modulates chemotaxis and activation of macrophages. Previous studies have shown that chemerin have both pro- and anti-inflammatory properties. Given the evidence that various inflammatory cytokines and chemokines are aberrant in endometriosis, chemerin may have biological relevance for the pathogenesis of endometriosis. Inflammation is an essential process in the development and growth of endometriosis. Chemerin is a recently found adipokine that modulates chemotaxis and activation of macrophages. Previous studies have shown that chemerin have both pro- and anti-inflammatory properties. Given the evidence that various inflammatory cytokines and chemokines are aberrant in endometriosis, chemerin may have biological relevance for the pathogenesis of endometriosis. DesignThis study was to examine the chemerin levels in serum and peritoneal fluid (PF) and chemerin mRNA expression from the ectopic and eutopic endometrium of women with or without endometriosis. This study was to examine the chemerin levels in serum and peritoneal fluid (PF) and chemerin mRNA expression from the ectopic and eutopic endometrium of women with or without endometriosis. Materials and methodsThe serum and PF samples were obtained from eighty women (30 women with endometriosis and 41 controls). The concentrations of chemerin in serum and PF from the subjects were determined by the enzyme-linked immunosorbent assay. The mRNA expression of chemerin in the ectopic and eutopic endometrium was measured using with real time RT-PCR. The serum and PF samples were obtained from eighty women (30 women with endometriosis and 41 controls). The concentrations of chemerin in serum and PF from the subjects were determined by the enzyme-linked immunosorbent assay. The mRNA expression of chemerin in the ectopic and eutopic endometrium was measured using with real time RT-PCR. ResultsThe mean concentrations of PF chemerin were significantly higher in women with endometriosis (4.17±0.1ng/mL) compared than those of the controls (3.5±0.1 ng/mL) (P<0.001). The serum levels of chemerin did not differ between the two groups. In analysis of real time RT-PCR with the ectopic endometrial tissue from the ovary (N=20) and normal eutopic endometrium of the controls (N=20), the chemerin mRNA expression was significantly higher in the ectopic endometrium compared than that in the eutopic endometrium of women without endometriosis (P=0.004). The mean concentrations of PF chemerin were significantly higher in women with endometriosis (4.17±0.1ng/mL) compared than those of the controls (3.5±0.1 ng/mL) (P<0.001). The serum levels of chemerin did not differ between the two groups. In analysis of real time RT-PCR with the ectopic endometrial tissue from the ovary (N=20) and normal eutopic endometrium of the controls (N=20), the chemerin mRNA expression was significantly higher in the ectopic endometrium compared than that in the eutopic endometrium of women without endometriosis (P=0.004). ConclusionWe observed that chemerin, a potential inflammatory mediator, significantly increased in endometriotic PF and was highly expressed from the ectopic endometrial cells. The results of this study suggest that chemerin may play an important role in the pathogenesis of endometriosis. We observed that chemerin, a potential inflammatory mediator, significantly increased in endometriotic PF and was highly expressed from the ectopic endometrial cells. The results of this study suggest that chemerin may play an important role in the pathogenesis of endometriosis.