Increased Angiopietin-2 expression, enhanced proliferation, migration and formation of blood vessels in hemangioma-derived stem cells

Abstract

Objective To investigate the effect of Angiopoietin-2 (Ang2) up-regulation on the biological function of hemangioma stem cells (HemSCs). Methods HemSCs was isolated and purified by enzyme digestion and immunomagnetic beads sorting. HemSCs was stably up-regulated by Ang2 by lentiviral transfection. The expression level of secreted Ang2 and vascular endothelial growth factor (VEGF) in HemSCs up-regulated by Ang2 was detected by enzyme linked immunosorbent assay (ELISA) method. EDU method The Trangswell method was used to observe the proliferation and migration ability of HemSCs cells up-regulated by Ang2. The tumor formation of nude mice was observed by single cell suspension. The tumorigenic ability of HemSCs was up-regulated by Ang2. The expression of key target proteins in phosphatidylinositol 3 kinase (PI3K)/serine-threonine protein kinase (Akt) cell signaling pathway was detected by Western blotting. Results The proliferation of HemSCs cells up-regulated by Ang2 was enhanced (blank group: 117.000±3.786, control group: 126.000±3.464, experimental group: 179.667±2.603, P<0.01), and migration ability was enhanced (blank group: 102.667±2.028, control group: 108.000±2.646, experimental group: 308.667±2.603, P<0.01); Ang2 in HemSCs Ang2↑ group (blank group: 60.103±0.361, control group: 69.343±0.526, experimental group: 1 962.440±43.205, P<0.01), VEGF (The blank group: 92.667±3.480, the control group: 91.000±1.523, the experimental group: 455.000±15.100, P<0.01) the expression of secreted protein was up-regulated; PI3K-Akt signaling pathway-related protein: Akt1 (blank group: 0.612±0.163, control group: 0.624±0.163, experiment Group: 0. 907±0.162, P<0.01), PI3K (blank group: 0.624±0.163, control group: 0.474±0.163, experimental group: 0.766±0.162, P<0.01), PI3KR5 (blank group: 0.505±0.163, control group: 0.405±0.163, Experimental group: 0.890±0.162, P<0.01), PAkt (blank group: 0.549±0.163, control group: 0.644±0.163, experimental group: 0.684±0.162, P<0.01), p38 (blank group: 0.618±0.163, control Group: 0.759±0.163, experimental group: 0. 947±0.162, P<0.01) Enhanced expression in HemSCs Ang2↑ group. Conclusion Upregulation of Ang2 can enhanced HemSCs proliferation, migration, in vivo angiogenesis; through PI3K-Akt signaling pathway Development has an impact. Key words: Hemangioma; Stem cell; Ang-Tie2 lentivirus; Signaling pathway