Abstract
Tilapia (Oreochromis mossambicus) were divided into three groups: control (injected with saline and handling stressed), blank (handling stressed alone) and experimental group (injected with cadmium and handling stressed). Cortisol and metallothionein (MT) were determined in order to approach the inducing effect of MT by cortisol. Results showed cortisol level in experimental and control group was higher than blank group at 6 h after injection. Besides, it was significantly higher in experimental group than control and blank group at 48 h after Cd-injection. MT content was significantly higher during 6 h and 48 h than 0 h upon Cd-injection, but it wasn't showed on both of control and blank group except at 48 h. These data demonstrated the levels of cortisol were revealed at 6 h after injected-stress, the highest levels of cortisol were secreted at 12 h in this three groups and recovery after 48 h except experimental group. We suggested that MT content increase might be as an indicator of late stress response, but the major role of MT was greater appearing upon heavy metal treatment.
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