Increase in total protein following infection of CV-1 cells with SV40 virus as assayed by flow cytometry

Abstract

The changes in cell size and total protein were determined for G1-arrested, contact-inhibited CV-1 cells infected with Simian virus 40 (SV40). The assays used were the Biorad total protein assays (Bradford and DC protein assays) on a standard number of cells, total protein as assayed by fluorescein isothiocyanate (FITC) and SR101 by flow cytometry, orthoganol (90 degrees) light scatter by flow cytometry, and direct microscopic measurement with an ocular micrometer. Uninfected CV-1 cells and two cell lines with variations in DNA content (diploid vs. tetraploid) were used as controls for the studies presented. The results demonstrated a 40-60% increase in total protein at 32 to 42 h postinfection. These increases were similar to values obtained due to cellular changes resulting from viral replication and cell death.