Abstract
AbstractA unique method was used to synthesize extremely stable silver stearate nanoparticles (AgStNPs) incorporated in an organic-based monolith. The facile strategy was then used to selectively isolate hemeproteins, myoglobin (Myo) and hemoglobin (Hb). Ethyl alcohol, silver nitrate, and stearic acid were, respectively, utilized as reducing agents, silver precursors, and capping agents. The color changed to cloudy from transparent, indicating that AgStNPs had been formed. AgStNP nanostructures were then distinctly integrated into the natural polymeric scaffold. To characterize the AgStNP–methacrylate polymeric monolith and the silver nanoparticles, energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy were used. The results of the SEM analysis indicated that the AgStNP–methacrylate polymeric monolith’s texture was so rough in comparison with that of the methacrylate polymeric monolith, indicating that the extraction process of the monolith materials would be more efficient because of the extended surface area of the absorbent. The comparison between the FT-IR spectra of AgStNPs, the bare organic monolith, and AgStNP–methacrylate polymeric monolith confirms that the AgStNPs were immobilized on the surface of the organic monolith. The EDX profile of the built materials indicated an advanced peak of the Ag sequence which represented an Ag atom of 3.27%. The results therefore established that the AgStNPs had been successfully integrated into the monolithic materials. Extraction efficiencies of 92% and 97% were used to, respectively, recover preconcentrated Myo and Hb. An uncomplicated method is a unique approach of both fabrication and utilization of the nanosorbent to selectively isolate hemeproteins. The process can further be implemented by using other noble metals.
Highlights
The unique electrical, optical, mechanical, heat conduction, and catalytic features of metals have made them extremely noticeable in recent years [1,2,3,4,5]
The study aimed at fabricating integrated values of AgStNPs and the ones for methacrylate polymeric monolith within the micropipette mainly to enhance the significant features of the monolithic sorbent, so that it could be used for the extraction of the hemeprotein
The process of preparing the poly(ethylene glycol) diacrylate cryogels, which are composite and in capillary form that could be applied in the disconnection of the acknowledged proteins that are of the hydrophobic interaction chromatography mode (Arrua et al.) [55], takes place
Summary
The unique electrical, optical, mechanical, heat conduction, and catalytic features of metals have made them extremely noticeable in recent years [1,2,3,4,5]. The fine particle sizes of the silver nanoparticles make them significantly important in microelectronics and electronics especially because it has unique features like high opposition towards oxidation and high thermal and electrical conductivity. No study has reported the utilization of stearic acid as a capping agent during the silver nanoparticle synthesis when the water-phase system is used. Some of the approaches include the utilization of monomers that have specific functions [36] like working on chemical surfaces of the original monoliths [37], grafting polymerization [38,39,40,41], and the integration of the nanostructures of the polymeric scaffold [32,42,43]. The UV-vis spectrophotometer (GENESYS 10S) was purchased from Thermo ScientificTM (Toronto, ON, USA)
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