Abstract
One lake sediment and three soils for rice production were used to test the effectiveness of inhibiting of nitrous oxide (N2O) reduction to dinitrogen gas (N2) by acetylene (C2H2) using 15N tracer. Regardless of the sources of the samples, results show that in presence of C2H2, significant isotopic enrichment of 15N of N2 was found at end of a typical denitrification assay. The δ15N of N2 value increased from 0‰ to 7.8–19.3‰ and 7.5–10.6‰ for the treatment with addition of 0.05 and 0.2 mg 15N nitrate, respectively. Such 15N enrichment can be interpreted as N2 formation accounting for 15.3% and 2.5% of the total added N in these two treatments, respectively. Nitrous oxide accumulation in presence of C2H2 could not account for the total added N. The result indicates incomplete inhibition of N2O reduction to N2 by C2H2 in denitrification when N2O reduction enzyme is developed.
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