Inclusion Complex of 2-Methyl Mercapto Phenothiazine with Hydroxy Propyl β-Cyclodextrin: Characterization and Cytotoxic Activity Evaluation

Abstract

Abstract A host-guest inclusion complex of hydroxy propyl β-cyclodextrin (HBCD) and 2-methyl mercapto phenothiazine (2MMPT) was prepared and investigated in liquid state and in solid state. In liquid state, UV-visible and fluorescence spectroscopic methods are used to investigate the inclusion complex. The binding constant of 2MMPT: HBCD inclusion complex was calculated using Benesi-Hildebrand plot. The 1:1 stoichiometric ratio of inclusion complex was derived from Benesi-Hildebrand plot and Job’s plot of continuous variation method. The solid HBCD: 2MMPT inclusion complex was prepared by coprecipitation method and characterized using FT-IR, 1 H NMR, 13 C NMR, and ESI-MS analytical methods. The cytotoxicity of HBCD: 2MMPT inclusion complex was studied on human breast cancer cell line MCF 7 showed that the cell cytotoxicity of HBCD: 2MMPT inclusion complex was close to free 2MMPT and inclusion complexation with HBCD retained the anticancer activity of 2MMPT.