Abstract
The aim of this study was to investigate the presence of three AME-encoding genes and to determine the resistance profiles of clinical Acinetobacter baumannii and Pseudomonas aeruginosa isolates. To evaluate the susceptibility profile towards gentamicin and amikacin, the automated Vitek 2 Com-pact method (bioMérieux, Marcy-l' Etoile, France) was used. The presence of the aac(6')-Ib-cr, ant(3'')-Ia, and aph(3'')-Ia genes was analyzed in 35 A. baumannii and 63 P. aeruginosa isolates using PCR. Our investigation revealed diverse resistance profiles across the hospitals studied. Isolates of A. baumannii from Hospital 2 and P. aeruginosa from both hospitals exhibited significant resistance to the tested aminoglycosides, while A. baumannii isolates from Hospital 1 showed remarkable sensi-tivity. The aac(6')-lb-cr gene was detected in 55% of the isolates, ant-3''-la in 41%, and aph(3'')-la in 42%. This study reports the first occurrence of the aph(3'')-la gene in these two pathogens in Brazil. It was possible to correlate the phenotypic profile of anti-microbial sus-ceptibility with the genotypic profile of most isolates. These findings underscore the incidence and spread of these genes, high-lighting the need for surveillance to implement measures to control hospital outbreaks.
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