Inactivation of foodborne pathogens in powdered red pepper (Capsicum annuum L.) using combined UV-C irradiation and mild heat treatment

Abstract

This study was performed to investigate the effectiveness of combined ultraviolet (UV-C) irradiation and mild heating as an alternative to conventional inactivation of foodborne pathogens, including Escherichia coli O157:H7 and Salmonella Typhimurium on powdered red pepper. A cocktail of three strains of E. coli O157:H7 ATCC 35150, ATCC 43889, ATCC 43890) and S. Typhimurium (ATCC 19585, ATCC 43971, DT 104) was inoculated onto powdered red pepper and then treated with UV-C irradiation and mild heat. A constant UV intensity (3.40 mW/cm2) of the emitting lamps was applied to samples for 5, and 10 min at 25, 35, 45, 55, and 65 °C, respectively. Also, quality change of powdered red pepper was measured in order to identify the efficiency of combined treatment. The reduction levels of E. coli O157:H7 and S. Typhimurium on powdered red pepper when treated with UV-C irradiation alone at 20.4 kJ/m2 for 10 min was 0.22 and 0.29 log CFU/g, respectively. While, combined treatment with mild heating at 65 °C reduced the surviving numbers of each pathogens by 2.88 and 3.06 log CFU/g, respectively. Although the inactivation efficiency was influenced less by the UV-C radiation dose, the synergistic effect was observed with increasing temperature and UV-C radiation dose. CIE color value and extractable color value were not significantly (P > 0.05) different between non-treated and combination treated samples. The moisture and capsaicinoids contents showed significant (P < 0.05) differences when treated at 65 °C because of sample drying during heat treatment. Therefore, these results suggest that UV-C irradiation combined with mild heating can be utilized by the food industry in order to effectively inactivate E. coli O157:H7 and S. Typhimurium without incurring quality deterioration of powdered red pepper.