Inactivation, lysis and degradation by-products of Saccharomyces cerevisiae by electrooxidation using DSA.

Abstract

The yeast Saccharomyces cerevisiae, a microorganism with cell walls resistant to many types of treatments, was chosen as a model to study electrochemical disinfection process using dimensionally stable anodes (DSA). DSA electrodes with nominal composition of Ti/RuO2TiO2 and Ti/RuO2TiO2IrO2 were evaluated in 0.05molL-1 Na2SO4 containing yeast. The results showed inactivation about of 100% of the microorganisms at Ti/RuO2TiO2 by applying 20 and 60mAcm-2 after 120min of electrolysis, while a complete inactivation at Ti/RuO2IrO2TiO2 electrode was achieved after 180min at 60mAcm-2. When chloride ions were added in the electrolyte solution, 100% of the yeast was inactivated at 20mAcm-2 after 120min of electrolysis, independent of the anode used. In the absence of chloride, the energy consumption (EC) was of 34.80kWhm-3, at 20mAcm-2 by using Ti/RuO2TiO2 anode. Meanwhile, in the presence of chloride, EC was reduced, requiring 30.24 and 30.99kWhm-3 at 20mAcm-2, for Ti/RuO2TiO2 and Ti/RuO2IrO2TiO2 electrodes, respectively, The best performance for cell lysis was obtained in the presence of chloride with EC of 88.80kWhm-3 (Ti/RuO2TiO2) and 91.85kWhm-3 (Ti/RuO2IrO2TiO2) to remove, respectively, 92 and 95% of density yeast. The results clearly showed that yeast, as a model adopted, was efficiently inactivated and lysed by electrolysis disinfection using DSA-type electrodes.