Abstract
Background and Aim:The Aujeszky’s disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky’s disease based on “Kordai” virus strain.Materials and Methods:To test the inactivation of the “Kordai” strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD50/ml) which is causative of Aujeszky’s disease, next-generation teotropin and propolis preparations were usedin concentrations of 0.1%, 0.08%, and 0.04%.Results:As a result of comparative studies on the optimization of parameters for inactivating the “Kordai” virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID50/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells).Conclusion:To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.
Highlights
Aujeszky’s disease is an acute viral disease involving epizootic and sporadic cases
The aim of this study was to develop an inactivated vaccine based on the “Kordai” virus strain
Bartha K61 (e.g., Ingelvac®, Boehringer Ingelheim Vetmedica, USA Aujeszky MLV, an antigenic component labeled for the glycoprotein-attenuated Bartha K-61 strain of Aujeszky’s disease virus) induces an immune response to Aujeszky’s disease due to the causative virus 2-3 weeks after application
Summary
Aujeszky’s disease is an acute viral disease involving epizootic and sporadic cases. The disease is recorded among pigs, dogs, and cats. The causative agent of Aujeszky’s disease is Alphaherpesvirinae suid herpesvirus, a species of viruses belonging to the genus Varicellovirus, family Herpesviridae [1]. Infection is usually derived from sick animals and virus carriers. According to the International Epizootic Bureau, Aujeszky’s disease is the most economically and socially significant epizootic disease. The Aujeszky’s disease, known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky’s disease based on “Kordai” virus strain
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