Abstract
The aim of this work was to detect in vivo effects of cadmium on marine organisms Cerastoderma glaucum and to determine the optimal conditions for the metallothionein-like protein (MTLP) induction in this species under laboratory conditions. The cockles Cerastoderma glaucum were exposed in vivo to different cadmium concentrations (50, 75, 100 and 150 µg L−1). Stress on stress test, cadmium, metallothionein-like protein (MTLP), malondialdehyde (MDA) and protein levels were measured after 5, 10, 15 and 20 days of metal exposure. The results showed that the CdCl2 concentrations of 50 µg L−1 for 15 days as well as 150 µg L−1 for 10 days provoked the highest MTLP induction. Nevertheless, the results obtained by the MDA, protein quantification and the stress on stress test showed that animals exposed to 50 µg L−1 for 15 days were in a better health status than those exposed to 150 µg L−1 CdCl2 for 10 days.
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