Abstract

The effect of red (RP) and purple (PP) Pitanga (Eugenia uniflora) L. spray dried and freeze dried powders on modulating stress response signaling was evaluated in transgenic C. elegans carrying promoter‐GFP constructs for several genes in the IGFR/PI3K/AKT, ARE/NRF2, Sir2.1/AMPK, mTOR/HIF‐1α, apoptosis/cell cycle and UPR signaling pathways. Relative fold changes in gene expression in response to pitanga treatments were quantified flurometrically. Neuroprotective effects of RP and PP in experimentally induced neurodegeneration were evealuated in C. elegans models for Alzheimer’s disease (AD) and Parkinson’s disease (PD). For AD, time to thermally induced Aβ1‐42 aggregation mediated paralysis was evaluated in transgenic C. elegans (CL4176). For PD, MPP+ induced neurodegeneration was qualtified by loss in motility due to paralysis. Results suggest an upregulation in ARE and Sir2.1 signaling in response to Pitanga treatment. Treatment with polar acidic, polar basic and polar neutral fractions, abrogated Aβ1‐42 induced paralysis and MPP+ induced neurodegeneration for PD by 15‐22%. The processing technology used to produce Pitanga powders significantly impacted bioactivity.Grant Funding Source: Supported by: Vrdür Foundation (USA) & CAPES (Brazil)

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