In vivo sedative and muscle relaxants activity of Diospyros lotus L

Abstract

ABSTRACT Objective To evaluate the sedative effect of Diospyros lotus L ( D. lotus ) extract in mice using the open field and Rota rod tests. Methods For the sedative and muscle relaxants activities of extract/fractions of the plant, in-vivo open field and phenobarbitone-induced sleeping time were used, while the Roda rod test was employed in animals for the assessment of muscle relaxant activity. Results Results from this investigation revealed that the extracts of D. lotus have exhibited significant sedative effect in mice (45.98%) at 100 mg/kg i.p . When the extract was partitioned with different solvents, the n -hexane fraction was inactive whereas the chloroform fraction was the most active with 82.67% sedative effect at 50 and 100 mg/kg i.p. On the other hand, the ethyl acetate and n -butanol fractions displayed significant sedative effects (55.65% and 40.87%, respectively) at 100 mg/kg i.p. Among the tested extract/fractions, only chloroform and ethyl acetate fractions showed significant ( P Conclusions In short, our study provided scientific background to the traditional uses of D. lotus as sedative.