Abstract
Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m(-3) for one week). Gallic acid at 10 mg kg(-1) and 20 mg kg(-1) and vitamin C for positive controls (10 mg kg(-1)) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8%) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4%, 63.69%, and 42% (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.
Highlights
Seyed Fazel NABAVI1, Solomon HABTEMARIAM2, Antoni SUREDA3, Akbar HAJIZADEH MOGHADDAM4, 5, Maria DAGLIA6, and Seyed Mohammad NABAVI1
Thiobarbituric acid reactive substances, antioxidant enzyme activities, and the level of reduced glutathione were evaluated in rat erythrocytes
In order to continue our work on P. peltatum and validate the therapeutic potential of gallic acid from natural sources, the present study was designed to study the in vivo protective effect of gallic acid against oxidative damages in rat erythrocytes
Summary
Seyed Fazel NABAVI1, Solomon HABTEMARIAM2, Antoni SUREDA3, Akbar HAJIZADEH MOGHADDAM4, 5, Maria DAGLIA6, and Seyed Mohammad NABAVI1. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes. Recent studies have suggested that fluoride intoxication leads to the down-regulation of antioxidant enzymes [3], an increase in relative oxygen species (ROS), and oxidative stress [4]. The extracts of its leaves possess potent radical scavenging effects, protect biological macromolecular molecules (e.g., DNA) from oxidative damage, and protect cultured cells from hydrogen peroxide (H2O2)-induced cell death [9] Subsequent studies on this plant revealed active antioxidant components such as gallic acid and a number of other related polyphenols [12, 13]. In order to continue our work on P. peltatum and validate the therapeutic potential of gallic acid from natural sources, the present study was designed to study the in vivo protective effect of gallic acid against oxidative damages in rat erythrocytes
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