Abstract
PurposeTraumatic spinal cord injury (SCI) is a devastating condition which affects millions of people worldwide causing major disability and substantial socioeconomic burden. There are currently no effective treatments. Modulating the neuroinflammatory (NI) response after SCI has evolved as a major therapeutic strategy. PET can be used to detect the upregulation of the 18-kDa translocator protein (TSPO), a hallmark of activated microglia in the CNS. We investigated whether PET imaging using the novel TSPO tracer [18F]GE-180 can be used as a clinically relevant biomarker for NI in a contusion SCI rat model, and we present data on the modulation of NI by the lipid docosahexaenoic acid (DHA).MethodsA total of 22 adult male Wistar rats were subjected to controlled spinal cord contusion at the T10 spinal cord level. Six non-injured and ten T10 laminectomy only (LAM) animals were used as controls. A subset of six SCI animals were treated with a single intravenous dose of 250 nmol/kg DHA (SCI-DHA group) 30 min after injury; a saline-injected group of six animals was used as an injection control. PET and CT imaging was carried out 7 days after injury using the [18F]GE-180 radiotracer. After imaging, the animals were killed and the spinal cord dissected out for biodistribution and autoradiography studies. In vivo data were correlated with ex vivo immunohistochemistry for TSPO.ResultsIn vivo dynamic PET imaging revealed an increase in tracer uptake in the spinal cord of the SCI animals compared with the non-injured and LAM animals from 35 min after injection (P < 0.0001; SCI vs. LAM vs. non-injured). Biodistribution and autoradiography studies confirmed the high affinity and specific [18F]GE-180 binding in the injured spinal cord compared with the binding in the control groups. Furthermore, they also showed decreased tracer uptake in the T10 SCI area in relation to the non-injured remainder of the spinal cord in the SCI-DHA group compared with the SCI-saline group (P < 0.05), supporting a NI modulatory effect of DHA. Immunohistochemistry showed a high level of TSPO expression (38 %) at the T10 injury site in SCI animals compared with that in the non-injured animals (6 %).Conclusion[18F]GE-180 PET imaging can reveal areas of increased TSPO expression that can be visualized and quantified in vivo after SCI, offering a minimally invasive approach to the monitoring of NI in SCI models and providing a translatable clinical readout for the testing of new therapies.Electronic supplementary materialThe online version of this article (doi:10.1007/s00259-016-3391-8) contains supplementary material, which is available to authorized users.
Highlights
Traumatic spinal cord injury (SCI) is a devastating condition, affecting around 2.5 million people worldwide [1]
The BBB scores showed a severe locomotor impairment in SCI animals while normal locomotion was observed in the laminectomy only (LAM) and the naive animals
In the docosahexaenoic acid (DHA) study, treatment with DHA after injury led to a marked improvement in locomotor outcome: the SCI-DHA animals had a BBB score of 5.3 ± 0.6 compared with 1.8 ± 0.6 in the SCIsaline animals (P < 0.001; Table 1)
Summary
Traumatic spinal cord injury (SCI) is a devastating condition, affecting around 2.5 million people worldwide [1]. It has a substantial impact on individuals and carers, and is associated with a major socioeconomic cost [2]. MRI is currently considered the standard approach for the prognostication of acute SCI [8] and more recently, the use of functional nuclear imaging approaches has been providing new targets for assessing the inflammatory and metabolic changes in the central nervous system (CNS), providing new perspectives on the pathophysiology of neurotrauma [9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: European Journal of Nuclear Medicine and Molecular Imaging
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
