Abstract
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
Highlights
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders
The neural probe is maintained in the interface unit which is fixed to the stereotacticguided arm and can be precisely implanted in the targeted brain area
We demonstrated a method for in vivo acute neurosampling using an advanced neural probe system combined with a mass spectrometry (MS)-based detection method
Summary
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. A major advantage of this approach is to provide a high collection efficiency compared to the microdialysis approach which is capital for sampling low concentration neurochemicals This is mainly enabled by the direct contact of the perfusate with the ECF medium and the resulting fast diffusion occurring toward the collected solution, in contrast to the slow diffusion through a microdialysis membrane[15]. This approach is very promising since it could enable the rapid sampling of molecules of interest, Taylor dispersion which tends to spread the distribution of solute in a liquid flowing through a tube will distort the time-history of the sampling[17]. The configuration of the reading zone (sample target) is often associated with an xy-stage which makes a Brain fluid sample
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