In Vivo Imaging Guided GMBP1 Modified Nanoplatform for Real Time Monitoring and Photothermal Therapy of Multidrug Resistance in Gastric Cancer

Abstract

Chemotherapy for gastric cancer (GC) is easy to cause multidrug resistance. Accordingly, it is of great significance to accurately monitor multidrug resistance for individualized treatment in GC. Molecular imaging technology provides a powerful means for the detection of multidrug resistance in GC. Research suggests that GMBP1 can specifically combined with overexpressed GRP78 receptor on GC multidrug resistance cells. Therefore, we constructed a dual-mode imaging nanocomplex based on GMBP1 (Au@bMSN-Cy7.5-GMBP1) that enables real-time multidrug resistance monitoring In Vivo through fluorescence imaging and photoacoustic imaging. In addition, Au@bMSN-Cy7.5-GMBP1 has a good photothermal effect so that it can be used for In Vivo photothermal therapy of multidrug resistance of GC. Au@bMSN-Cy7.5-GMBP1 has good stability and photothermal effect and its particle size is about 100 nm. Au@bMSN-Cy7.5-GMBP1 has low cytotoxicity and can effectively inhibit tumor cell growth under laser irradiation. Au@bMSN-Cy7.5-GMBP1 has low hematotoxicity and good biosafety. Au@bMSN-Cy7.5-GMBP1 can be observed to accumulate gradually in the tumor site through fluorescence imaging and photoacoustic imaging, so as to realize real-time monitoring of GC multidrug resistance. In Vivo photothermal therapy results show that Au@bMSN-Cy7.5-GMBP1 can effectively inhibit tumor growth under laser irradiation, and realize photothermal treatment of multidrug resistance in GC. Au@bMSN-Cy7.5-GMBP1 provides an excellent nano-platform for individualized treatment and multidrug resistance monitoring of GC.