In Vivo GnRH Responsiveness of LH Secretion in the Female Turtle, Trachemys scripta, in Relation to the Reproductive Stage

Abstract

The effects of several forms of gonadotropin-releasing hormone (GnRH) on the in vivo secretion of luteinizing hormone (LH) were investigated in the female turtle Trachemys scripta. Serial injections of large vitellogenic turtles with 1-2 μg (ca. 0.15 μg/100 g turtle) of chicken GnRH-I (cGnRH-I) and multiple blood sampling revealed that mature females were unresponsive to GnRRH. In contrast, both forms of avian GnRH, cGnRH-I and cGnRH-II, and an avian GnRH analogue (GnRHa) significantly increased circulating levels of LH in previtellogenic turtles. In these turtles, plasma LH increased within 10 min after the initial injection of 0.5 μg GnRHa or cGnRH-I, but this increase in LH was generally transient and could not be sustained for more than 2 hr even with repeated GnRH injections. Turtles treated with GnRHa on Day 1 showed attenuated pituitary responsiveness to subsequent GnRH challenges on Day 2, whereas turtles treated with both native forms of chicken GnRH remained equally responsive on Day 2. In vitro, GnRHa was more resistant to degradation in plasma than both native forms of GnRH, and the clearance rate of GnRHa in vivo was also slower than that of cGnRH-I. The results show, for the first time, that in vivo GnRH administration stimulates and desensitizes LH secretion in the turtles, but the GnRH responsiveness is related to reproductive status. Furthermore, the results suggest that the desensitizing effects of various forms of GnRH in vivo may be related to their clearance rate.