GnRH-Induced Desensitization of in Vitro Luteinizing Hormone Secretion in the Turtle, Trachemys scripta

Abstract

Gonadotropin-releasing hormone (GnRH)-induced desensitization of pituitary luteinizing hormone (LH) secretion in female turtles (Trachemys scripta) was investigated by in vitro superfusion of whole or hemipituitaries with the endogenous forms of the peptide. In sub-adults, LH secretion was rapidly attenuated desensitized) by both prolonged and pulsatile treatments of chicken II GnRH (cII-GnRH), but pulsatile GnRH administration was more effective than prolonged administration in stimulating LH release. Prolonged administration of chicken I GnRH (cI-GnRH) also desensitized LH secretion; cI-GnRH was slightly less potent than cII-GnRH. GnRH-induced attenuation of LH secretion was not the result of tissue deterioration or depletion of relesable LH: GnRH responsiveness of desensitized pituitaries was fully restored after 4 hr of in vitro recovery, and further LH secretion was induced by tetraethylammonium chloride, a membrane depolarizing agent, in the desensitized glands; TRH-stimulated TSH release did not show desensitization in the same glands. Pituitaries from most adult (vitellogenic) females failed to respond to GnRH challenges; however, when responsive, adult pituitaries also desensitized under prolonged GnRH stimulation and were more resistant to GnRH-induced desensitization under pulsatile GnRH treatments. Current results show that adult T. scripta typically display lower GnRH sensitivity than the subadults; the latter are like mammals, birds, and goldfish in their susceptibility to desensitization by both forms of endogenous GnRH.