Abstract
AbstractAssay conditions in vivo for determination of nitrate reductase activity (NRA) in leaf and feeder root tissues of tea bushes (Camellia sinensis L.) were studied. The composition of the incubation mixture, pH, temperature, period of incubation and tea shoot components were varied. Maximum NRA was obtained in leaf with a medium containing 300 mg Polyclar AT with 300 mg leaf disc in 0.1 M phosphate buffer at pH 7‐5, 0.02 M KNO3 in a total volume of 5 ml and incubated for 4 hat 30°C in the dark. Propan‐1‐ol inhibited NRA in tea leaf and root. Highest activity was found in the first leaf.
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