Abstract
Maximum nitrate reductase (NR) activity was measured in two intertidal morphotypes of Zostera noltii (Hornem.) in Ria Formosa tidal lagoon, southern Portugal. The two morphotypes develop in the upper and lower limits of the intertidal meadows. The NR activity was measured using an in vivo method, without cell disruption. NR activity was 30–40 fold higher in leaves than in roots, which indicates that nitrate reduction is essentially made through the aerial part of the plant. The effects of assay temperature (5 °C steps, from 5 to 45 °C), pH (7, 8 and 9) and elevation (upper and lower intertidal) on leaf NR activity were tested in a factorial design ( n=5). Both elevation and assay temperature had a significant effect on NR activity, but not pH. NR activity was always higher in the upper intertidal plants, at all temperatures. Activity peaks for upper and lower plants were, respectively, 6.12 μmol NO 2 − g −1 DW 0.5 h −1 at 25 °C, and 3.30 μmol NO 2 − g −1 DW 0.5h −1 at 35 °C. Further investigation on environmental factors concerning the intertidal environment must be developed, as they are probably responsible for the significant differences found between the values of NR activity in the upper and lower morphotype.
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