In Vivo Antihypercholesterolemic Potential of Uncaria cordata (Lour.) Merr as Ethanolic Extract

Meiriza Djohari,Tri Wahyuni,Mira Febrina

doi:10.22146/mot.64531

Abstract

The present investigation aims to evaluate the antihypercholesterolemic potential of Uncaria cordata (Lour.) Merr. as an ethanolic extract in diet-induced hypercholesterolemic male white mice model. In this study, white mice were segregated into 6 groups; all the groups except the normal control group were given a high-fat diet to induce hypercholesterolemia. After induction of cholesterolemia, normal and negative control groups were treated with NaCMC, the positive control group was treated with atorvastatin, and the remaining three groups received ethanolic extract Uncaria cordata (Lour.) Merr. in three doses (100, 200, and 400 mg/Kg BW) for a treatment period of 29 days. Measurement of cholesterol levels was performed on days 0, 15, 22, and 29 using EasyTouch® GCU digital devices. The results were analyzed by one way ANOVA test and ANOVA Repeated test. The results showed that the ethanol extract of the Uncaria cordata (Lour.) Merr. root plant dose 100, 200, and 400 mg/Kg BW effect in lowering total cholesterol in male white mice significantly (P <0,05). Ethanol extract from Uncaria cordata (Lour.) Merr. a dose of 200 mg/Kg BW showed a better decrease in cholesterol levels on day 29 compared to day 22.

Highlights

Cholesterol is one of the plasma lipid compounds found in tissues in the form of plasma lipoproteins
Hypercholesterolemia is a condition that signed with Increased cholesterol levels in the blood and this is the main factor of atherosclerosis associated with coronary heart disease which can lead to many cases of death (Muhtadi et al, 2013)
The method used in this study is Point of Care Testing (POCT) (Point Of Care Testing), using an Easy Touch® tool equipped with a blood cholesterol test strip

Summary

INTRODUCTION

Cholesterol is one of the plasma lipid compounds found in tissues in the form of plasma lipoproteins. Based on research conducted by Frinanda (2014), the best dose of Uncaria gambir in inhibiting the increase in total cholesterol and stabilizing the blood value of mice is 50 mg/kg BW. Based on the above description, the researchers were interested in conducting a study on the effect of ethanol extract of leaves of Kaikkaik Root plants at doses of 100, 200, and 400 mg/kg BW in reducing levels of the total cholesterol of male white mice (Mus musculus L.). The method used in this study is POCT (Point Of Care Testing), using an Easy Touch® tool equipped with a blood cholesterol test strip. This method is easy for daily use and still gives accurate data. The selection of ethanol solvents for extraction in this study is based on the fact that besides being nontoxic, ethanol is a universal solvent so that it can attract polar and nonpolar compounds, the compounds expected to be attracted by these solvents are flavonoids (Marjoni, 2016)

METHODOLOGY

Methods

AND DISCUSSION

Findings

CONCLUSION