In vivo and in vitro healing potential of aqueous extract ointment Ageratum conyzoïdes Linn. applied to excision and incision wounds induced in Wistar rat and human cells

Abstract

The healing effect of the aqueous extract and ointment at 4 and 8 g/kg doses of Ageratum conyzoides Linn. on tracing and excision-induced wounds in the Wistar rat was the subject of our study. We used the classical tests described by some authors to carry out pharmacological work and organoleptic and toxicological analyses of the extract and formulation of this plant. The results, derived from this methodology, show that this ointment is homogeneous, weakly aromatic and acidic (pH : 5,6), rich in dissolved and mineral substances, no lethality was observed up to the dose of 3200 mg/kg in acute. Also, the aqueous extract of this plant has an abundance of Flavonoids. In addition, the healing effect of Ageratum conyzoides L. ointment was evaluated on deep wounds in Wistar rats. The planimetry results and measurements made over twentysix (26) days proved that the ointment at 4 and 8 g/rat weight; Madecassol® at 400 mg/kg (reference product) significantly reduced the mean area of the deep wounds in Wistar rats compared to the negative controls. In addition, the aqueous extract of Ageratum conyzoides L. at the dose of 5,5 ml/kg administered in vitro on cell model wounds created by scraping the cell web, significantly reduces the wound in 24 hours. In addition, at the dose of 11 ml/kg this scarring is complete, there is no visible wound trace. This reflects the presence of a cell thrust at the level of the scratch compared to the control lot, whose wound remained almost gaping. Thus, are confirmed the data of literature and traditional medicine on spa summarized efficacy.