Abstract
Objective: The objective the present work was to investigate in vivo and in vitro anti-diabetic potentials of methanol extract of Tephrosia calophylla against alloxan-induced diabetes in albino rats.Methods: For in vivo evaluation, diabetes was induced in albino rats by administering a single dose of alloxan. The study was designed to test the acute effect of methanol extract of Tephrosia calophylla (TCME) to reduce blood glucose in OGTT. The chronic study of 21 d was performed against diabetic rats and blood glucose was determined at 1st, 7th, 14th and 21st day. In chronic in vivo study, serum concentrations of insulin, urea, creatinine, total cholesterol, triglycerides, ALT and AST were also estimated at 21st day. The in vitro α-glucisidase inhibitory activity and α-amylase inhibitory activity were performed and IC50 values of the extract was determined. The glucose uptake by rat hemidiaphragm model was also used test potentials of the extract to increase utilization of the glucose by tissues.Results: In OGTT, standard glibenclamide and TCME at 400 mg/kg treated animals have shown significant reduction in blood glucose at 90 min but at 120 min, blood glucose level (BGL) was significantly reduced in glibenclamide and TCME at 200 mg/kg and 400 mg/kg treated animals compared to diabetic control group. In chronic modelthe methanol extract effectively reduced blood glucose levels (P<0.001) at 14 th and 21st day of study in therapeutic groups and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduce concentrations of SGOT, triglycerides (TGs), Total cholesterol (TC) and urea in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial effects of the extract in diabetes. The change in concentrations of SGPT and urea were less significant (P>0.01). In vitro studies, against both glucosidae and amyalase inhibitory activities, extract has shown significant IC50 values and it also enhanced glucose utilization by rat hemidaphragm.Conclusion: The results obtained from the present study suggest that, the methanol extract of Tephrosia calophylla possess significant in vivo anti-diabetic properties against alloxan induced diabetes in rats. The results also suggests that, TCME also possess the significant in vitro anti-dabetic potentials.
Highlights
Diabetes mellitus is a pathological condition characterized by metabolic alterations leads to hyperglycemia and other complications due to defects in secretion of insulin, action or both
Treatment withTCME (0.1% w/v) elicited a significant increase (p
We evaluated methanolic extract of Tephrosia calophylla leaves for the enhancement of insulin secretion and ability to lower the blood glucose level in diabetic rats by estimating the concentration of insulin in the blood
Summary
Diabetes mellitus is a pathological condition characterized by metabolic alterations leads to hyperglycemia and other complications due to defects in secretion of insulin, action or both. It has already been established that chronic hyperglycemia of diabetes is associated with long term damage due to insulin resistance or defect in release of insulin from pancreas [1, 2] It has an adverse effect on carbohydrate, lipid and protein metabolism resulting in chronic hyperglycemia and abnormality of lipid profile. In spite of the introduction and extensive utilization of hypoglycemic agents, diabetes and the related complications continue to be a major health problem worldwide, which is affecting nearly 10% of the population all over the world [4] In this regard medicinal plants continue to be an important therapeutic aid for alleviating ailments of humankind such as diabetes mellitus due to their less or no adverse effects. Study had been conducted to determine in vitro and in vivo anti-diabetic potentials of methanol extract of areal parts of Tephrosia calophylla
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