Abstract
Both in vivo and in vitro 31P-NMR spectroscopy were used to demonstrate metabolic changes in rat liver as a function of time after exposure to either carbon tetrachloride (CCl 4) or bromotrichloromethane (BrCCl 3). The inorganic phosphate resonance, measured in vivo, moves upfield, which is associated with a decrease in cytosolic pH over a 12 or 20 h period (for BrCCl 3 or CCl 4, respectively). Intoxication by CCl 4 or BrCCl 3 causes an intracellular acidosis to pH 7.05 or 6.82 (±0.05), respectively. Also, it has been found that halocarbon exposure increases the amounts of phosphomonoesters (PME) detected. High resolution in vitro 31P-NMR spectroscopy studies of perchloric acid extracts of CCl 4-treated rat livers indicated a significant increase in the height of the phosphocholine resonance in the PME region 4–5 h after CCl 4 exposure.
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