Abstract
In vitro multiplication of elite clones is an attractive approach in order to meet the requirement of quality propagules at large scale for commercial cultivation. In vitro maintenance is effective in maintaining the male sterile line. Standardized protocol was followed for in vitro maintenance of male sterile line. Ridge gourd genotypes were collected from different parts of the country and evaluated for variability with respect to fourteen traits. Sterility was assessed on the basis of pollen fertility percentage, which was found to be zero throughout the flowering season. There was no observable difference with respect to vegetative growth or production of female flowers between male sterile and fertile plants. In vitro regenerated plants exhibited stable male sterility all round the flowering season. Pollen fertility was found to be zero in all the male sterile plants. As compared to back crosses, three way crosses expressed early emergence of first male flower. F2 data indicated cytoplasmic genetic male sterility with more than one gene involved in fertility restoration. Male sterile plants can be used for commercial seed production in vegetable crops with less cost of cultivation.
Published Version
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