Abstract
Vanda tricolorLindey var.Suavisis an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of theV. tricolorMerapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is namedVanda tricolorvarSuavis‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize theHSP70gene in it. Methods: Standardin vitroculture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that > 90 % ofV. tricolor‘Queen Maxima’ seeds germinated well in all media and the best on VW medium.V. tricolorhas the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from thePhalaenopsis equestrisorchid, which is probably the reasonV. tricolorbecome superior to high temperature environments.
Highlights
Use Orchids (Orchidaceae) are flowering plants with the largest number of members about 26 972 species [1]
This study aims to determine the morphological and molecular characters of V. tricolor var Suavis, especially the character of the HSP70 gene in these plants
Based on morphological observations on Vanda tricolor that used as self-pollinated parent of V. tricolor to produce V. tricolor 'Queen Maxima', V. tricolor has a perennial, epiphytic, monopodial herbaceous plant habits (Figure 1A)
Summary
Use Orchids (Orchidaceae) are flowering plants with the largest number of members about 26 972 species [1]. Indonesia is one of the countries contributing to the diversity of orchids in the world, with around 5 000 species in Indonesia [2]. Orchids are ornamental plants with high commercial value [3]. One species of orchid that has high potential to be developed is Vanda tricolor Lindley var. V. tricolor has been reported to have a special character in the HSP70 gene structure. The 70-kDa heat-shock genes (HSP70s) encode a group of conserved chaperone proteins that play central roles in cellular networks of molecular chaperones and folding catalysts across all the studied organisms including bacteria, plants and animals [6]. The HSP70 gene is responsible for high temperature resistance [7]
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