In vitro Therapeutic Effect of Spirulina Extract

Abstract

Recent Research focused on bio therapeutic has wide range of application in medicinal field due to its activity and enormous nutritional values. The main objective of the study is to evaluate the blue green algae, Spirulina for their anti-bacterial, antioxidant, hemolytic, anti-diabetics and screening of phytochemical constituents. By disc diffusion method the four extracts (chloroform, hexane, petroleum ether and acetic acid) of Spirulina were screened for antibacterial activity against four pathogenic micro-organisms. The antioxidant activity was determined by DPPH method (free radical scavenging activity). The hemolytic activity was determined by using ELISA plate assay. In-vitro anti diabetic activity (Alpha amylase enzyme inhibition activity) of potential phytochemical constituents was determined qualitatively. The acetic acid extract of Spirulina showed significant anti-microbial activity among the different extracts tested. The most susceptible microorganism was found to be gram positive bacteria (Staphylococcus sp) and gram negative bacteria (E. coli, Klebsiella sp and Pseudomonas sp). DPPH free radical scavenging activity of Spirulina was found to be increase with its concentration. 2.303 ± 0.5 microgram of Spirulina was found in 20% concentration of ethanol extract. Spirulina was up to inhibit the activity of alpha-amylase that was dose dependent and also exhibits anti-inflammatory activity. Hence the present study shows that all the extracts of blue green algae of Spirulina have wide range of therapeutic effects.