In vitro synthesis of particulate glycogen from uridine diphosphate glucose

Abstract

High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor. The product obtained did not differ significantly from the native glycogen as judged by iodine spectrum, sedimentation coefficient in sucrose gradients, and by the effect of treatment with acid or alkali. Glycogen obtained from uridine diphosphate glucose differed from that prepared with glucose 1-phosphate as glucosyl donor.