A comparative study of glycogen and lactate formation from glucose, glucose 6-phosphate, α-glucose 1-phosphate, and uridine diphosphate glucose by intact novikoff ascites-hepatoma cells

Abstract

Novikoff ascites tumor cells form significant amounts of glycogen from glucose and uridine diphosphate glucose (UDPG). Glucose 6- and α-glucose 1-phosphates are poorly metabolized by freshly prepared tumor cells. Aging of the tumor cells (24 hours at 0–5 °) leads to a loss in glycogen formation and an increase in lactate production from glucose. Aged tumor cells also form significant amounts of lactate from glucose phosphates. Dinitrophenol is inhibitory to glycogen synthesis from glucose, less so with glucose phosphates as substrates, and has no effect when UDPG is the glycogen precursor. Glucose 6-phosphate stimulates glycogen synthesis from glucose and UDPG, while AMP reduces glycogen formation from UDPG and activates the reaction when glucose phosphates are the substrates.