Abstract
Aim: The aim of this study was to evaluate the suitability of Sensititre YeastOne (SYO) method for susceptibility profiles’ determination of non- Aspergillus moulds and of E-test for Aspergillus spp. and non- Aspergillus moulds, in comparison with the M38-A reference broth microdilution (BMD) method. Material and Methods: A total of 33 clinical isolates of filamentous fungi were tested. Results: The agreement between E-test and BMD at ± 2 dilutions was 82.4%, 83.3% and 82.4% for amphotericin B, itraconazole and voriconazole, respectively. The agreement between SYO and BMD at ± 2 dilutions was 76.5%, 66.7% and 88.2% for amphotericin B, itraconazole and voriconazole, respectively. The majority of differing results are due to higher MICs with the reference method. In particular, SYO was unable to detect some of the potentially amphotericin B resistant strains. We found that both E-test and SYO method were reproducible and served as suitable methods for antifungal susceptibility testing of moulds. Conclusion: In conclusion, both E-test and SYO method are promising, but require further investigation to identify the optimum conditions for their use in testing of susceptibility profiles of filamentous fungi to antifungal agents.
Highlights
The impact and severity of fungal infections have grown in recent decades and involve a wide range of fungal pathogens as etiological agents
Sensititre YeastOne (SYO) was unable to detect some of the potentially amphotericin B resistant strains. We found that both E-test and SYO method were reproducible and served as suitable methods for antifungal susceptibility testing of moulds
In conclusion, both E-test and SYO method are promising, but require further investigation to identify the optimum conditions for their use in testing of susceptibility profiles of filamentous fungi to antifungal agents
Summary
The impact and severity of fungal infections have grown in recent decades and involve a wide range of fungal pathogens as etiological agents. During this period, the number of immunocompromised patients has markedly increased [1]. The management of fungal infections is affected by the ability to carry out rapid and effective etiological diagnosis and availability of antifungal agents with proper spectrum of activity [3]. Susceptibility Testing of Aspergillus and Non-Aspergillus Filamentous Moulds to Antifungal Agents associated with invasive fungal infections [4], whereas early intervention with antifungal drugs may result in more effective management of high-risk patients [5]
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