Abstract
The aim of this study was to evaluate in vitro antimicrobial activity of 4 antibiotic agents (for E.faecalis) and 4 antifungal agents (for C.albicans) by agar dilution method. Additionally, modified strip diffusion method was used for detection of in vitro antimicrobial activities of 5% NaOCl, 2.5% NaOCl, 17% EDTA and 2% CHX and agar diffusion method for detection of in vitro susceptibilities of three intracanal medicaments for 18 E.faecalis and 18 C.albicans isolates from primary and secondary root canal infection. Isolates were recovered from 231 endodontic samples of patients, with the need of root canal treatment and retreatment. All tested E.faecalis isolates showed resistance to antibiotics. For irrigation solutions, 2% CHX was more effective in eliminating E.faecalis but 5% NaOCl showed larger inhibition zone than 2.5% NaOCl, 17% EDTA and 2% CHX. For intracanal medication, Ca(OH)2-CHX worked efficiently in killing E.faecalis isolates compared to Ca(OH)2-Steril saline solution, Ca(OH)2-Glycerin. For C.albicans, 18 isolates were susceptible to amphotericin B, nistatin, fluconazole but showed resistance to ketoconazole. 5% NaOCl was more effective in eliminating and produced larger inhibition zone compared to 2.5% NaOCl, 17% EDTA and 2% CHX. Ca(OH)2-Glycerin intracanal medication was better in eliminating C.albicans isolates and produced larger inhibition zone compared to other Ca(OH)2 medicaments. Key words:E.faecalis, C.albicans, antimicrobial, antibiotic, antifungal.
Highlights
The goal of endodontic treatment is the elimination of microbial infection in the root canal system by adequate instrumentation, irrigation and high technical quality of permanent root canal filling
The aim of the present investigation was to evaluate the antimicrobial effectiveness of endodontic medicaments against E.faecalis and C.albicans strains that have been detected in primary and secondary root canal infection
Infections of endodontic origin are treated without antibiotics because there is no blood circulation within a necrotic and infected pulp so antibiotics can’t reach and eliminate microorganisms present in the root canal system [14]
Summary
The goal of endodontic treatment is the elimination of microbial infection in the root canal system by adequate instrumentation, irrigation and high technical quality of permanent root canal filling. E.faecalis can grow at pH 9.6 and tolerate pH levels as high as 11.9; so they can withstand harsh enviromental conditions This makes these bacteria able to survive in the root canal as a single organism or as a major component of the flora [4]. Used antifungal agents may be useful in the treatment of root canal yeast infection, alone or in combination with a disinfectant [13]. The aim of this study was (a) to evaluate in vitro activity of four antibiotic and four antifungal agents using the agar dilution method (b) to evaluate the in vitro antimicrobial activity of 5% NaOCl, 2.5% NaOCl, 17% EDTA and 2% CHX solution by strip diffusion method (c) the in vitro susceptibility of three intracanal medicaments by agar diffusion method to E.faecalis and C.albicans isolates from primary and secondary root canal infection
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