Abstract
Aim: The aim of this study was to determine the frequency of two important pathogenic microorganisms associated with endodontic infections, Enterococcus faecalis and Candida albicans, in root canal samples from patients with necrotic pulps or failed canal therapy by polymerase chain reaction method. Method: Microbial samples were obtained from 117 teeth with necrotic pulp tissues and 114 teeth with failed endodontic treatment. Results: E.faecalis were identified in 16% of the necrotic and 10% of the retreated root canal infections by PCR. C.albicans genome were identified in 20% and 11% of the necrotic and retreated root canal infections, respectively, by PCR. The frequencies of microbiota were not statistically different between necrotic and retreatment groups (p > 0.05, chi squared test). Conclusions: PCR analysis of teeth with periapical lesions revealed that E.faecalis was found in fewer patients than in previous studies. The C.albicans prevelance was consistent with previous reports. No statistical difference was found between primary and secondary root canal infections for C.albicans or E.faecalis. Key words:Primary root canal infection, secondary root canal infection, E.faecalis, C.albicans.
Highlights
The most important factor associated with endodontic failure is the presence of persistent microbial infection in the root canal system [1]
E.faecalis can occur in primary root canal infections, especially in teeth with coronal leakage, typically in low numbers [7] and is the most common organism cultured from failed root canal therapy, with 12–90% prevalence [8,9]
We have investigated the presence of E.faecalis and C.albicans in primary and secondary infected root canals
Summary
The most important factor associated with endodontic failure is the presence of persistent microbial infection in the root canal system [1]. E.faecalis can occur in primary root canal infections, especially in teeth with coronal leakage, typically in low numbers [7] and is the most common organism cultured from failed root canal therapy, with 12–90% prevalence [8,9]. This disparity in the composition of root canal microbiota can be related to differences in methodologies of studies, but may be influenced by other factors, such as geographical effects [10]. Candida species are known to be present in the oral cavity [16] and C.albicans is the most commonly isolated fungal species in the oral cavity (30–45% )
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