Abstract
For conception and the development of healthy embryos, sperm DNA integrity is crucial. According to a growing body of studies, there is a strong correlation between sperm DNA damage and male infertility. Among the new medicines being developed in the medical field, the application of Platelet Rich Plasma (PRP) in human reproduction has yet to be examined. A total of 100 semen samples were used in the current experimental investigation. From November 2020 to June 2021, the research was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Masturbation was used to get an ejaculated semen sample. After semen analysis, the samples were separated into two equal parts, one without autologous PRP and the other with 2% autologous PRP, with the DNA fragmentation assessed using the Sperm Chromatin Dispersion Test. There was highly significant reduction in DNA fragmentation index (p < 0.001). The mean sperm DNA integrity was reduced after adding PRP (33.85±16.73 vs 38.55±16.64), Mean (± SE). PRP has been shown to improve human sperm DNA integrity.
Highlights
After one year of regular, unprotected sexual contact, infertility is defined as a couple's inability to conceive (Zegers-Hochschild, et al [1])
A normal semen analysis is found in roughly 15 % of infertile patients (Agarwal and Allamaneni [4]). on the other hand, Sperm concentration, motility and morphology, might not adequately reflect sperm DNA integrity (Guzick, et al [5]), which is harmful to embryo development, normal fertilization and assisted reproductive technology (ART) success (Simon, et al [6])
The current study's aims -in this regard- were to be one of the few that looked at the effects of Platelet Rich Plasma (PRP) on a variety of sperm functionality characteristics before looking into the possible influence of PRP on human sperm DNA fragmentation for the first time
Summary
After one year of regular, unprotected sexual contact, infertility is defined as a couple's inability to conceive (Zegers-Hochschild, et al [1]). Traditional semen analysis is still used to assess infertile men, despite the fact that it does not accurately predict male fertility or the success of assisted reproductive technology (ART), it is widely used. On the other hand, Sperm concentration, motility and morphology, might not adequately reflect sperm DNA integrity (Guzick, et al [5]), which is harmful to embryo development, normal fertilization and ART success (Simon, et al [6]). Platelets are megakaryocyte derivatives with α granules containing many secretory proteins. They are members of the growth factor, cytokine, and chemokine families, through growth factors, and their dense granules are involved in the acceleration and control of wound healing processes. It is recommended that the platelet concentration in the PRP be 3 to 5 times higher than normal. PRP's positive effects are mostly due to its different bioactive components. (Magalo, et al [11])
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