Abstract
Objective: The current study is an attempt to screen for the in vitro clot lysis and proteolytic activity of aqueous extract of Leucas aspera leaves.
 Methods: Thrombolytic activity and protease activity of the crude enzyme obtained by ammonium sulfate precipitation and dialysis were assayed using blood clot and casein as substrates respectively. Native PAGE and gel documentation studies were performed to calculate the molecular weight of the enzyme.
 Results: In the study, 40% salt fractioned crude enzyme sample exhibited significant thrombolytic and caseinolytic activity. Further dose-dependent increased activity was observed with the maximum lytic activity of 52.11±1.04 % at 1 mg/ml of the sample when compared to the reference drug streptokinase (71.39±0.32%). Also, 68.72±0.62 U/hr of caseinolytic activity was observed for 1 mg/ml of the sample fraction.
 Conclusion: The study highlights and validates the efficacy of Leucas aspera leaves extract for thrombolytic and proteolytic actions. Enzyme with an approximate molecular weight, 19.89 KDa could be responsible for the significant lytic activity.
Highlights
Blood clot developed in the circulatory system due to the failure of homeostasis causes vascular blockage and while recovering leads to serious consequences in atherothrombotic diseases [1,2,3]
Thrombolytics are used to dissolve the fibrin of blood clots, which are potentially lifethreatening, especially those in the arteries of the heart and lungs
The current study aims at determining the thrombolytic and proteolytic activities of Leucas aspera leaves extract
Summary
Blood clot (thrombus) developed in the circulatory system due to the failure of homeostasis causes vascular blockage and while recovering leads to serious consequences in atherothrombotic diseases [1,2,3]. Thromboembolic disorders such as myocardial infarction, cerebrovascular thrombosis, pulmonary embolism, and venous thrombolism are life-threatening for human beings [4]. Thrombolytics are used to dissolve the fibrin of blood clots, which are potentially lifethreatening, especially those in the arteries of the heart and lungs. Such changes occurred since natural medicine or traditional medicine made a revolutionary come-back with renewed strength and vigor to play a more significant role in the management of human health [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
