In vitro study of anthelmintic effect of butterfly pea (Clitoria ternatea) flower aqueous extract on Tubifex tubifex

Abstract

Clitoria ternatea (CT) or butterfly pea was one herbs used for Ayurvedic and other traditional medicines. Utilization of its roots, flowers, and leaves proposed several medical adventages with anthelmintic was one of it. Among current anthelmintic burden of resistance, this study aimed to examine the anthelmintic profile from CT flower aqueous extract on Tubifex tubifex. The study was done by conducting a pilot experimental study by extracting CT flowers to water and piping the extracts of different level of dilution to several group of pots containing Tubifex tubifex. Anthelmintic activity was determined with its paralyzing effect and was compared to negative and positive controls with levamisole. Phytochemicals substances were detected with alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids detected. The extract exerts anthelmintic activity it 1:4, 1:40, and 1:400 dilutions and was comparable to 1mg/mL and 0.1mg/mL levamisole. Time to paralysis observed suggested a dose-response relationship of the extract on its anthelmintic activities. It was understood the phytochemicals influents the anthelmintic activity by paralysis worms and leads to death. It was concluded that CT flower aqueous extract presents anthelmintic activity, with further experimental study will needed to be conducted.