In vitro studies on the inhibition of α-amylase and α-glucosidase by hydro-ethanolic extract of Pluchea lanceolata, Alhagi pseudalhagi, Caesalpinia bonduc

Abstract

Pharmacognosy Research,2019,11,3,310-314.DOI:10.4103/pr.pr_31_19Published:August 2019Type:Original ArticleAuthors:Anupam Kumar Sachan, Ch. V. Rao, and Nikhil Kumar Sachan Author(s) affiliations:Anupam Kumar Sachan1, Ch V Rao2, Nikhil Kumar Sachan3 1Department of Pharmaceutics, Dayanand Dinanath College, Institute of Pharmacy, Kanpur, Uttar Pradesh, India. 2Department of Pharmacology, Principal Scientist, National Botanical Research Institute, Lucknow, Uttar Pradesh, India. 3Education Officer, University Grants Commission, New Delhi, India. Abstract:Background: Pluchea lanceolata (Rasna), Alhagi pseudalhagi (Jawasa), and Caesalpinia bonduc (Latakaranja) important medicinal plants widely used in India as folk medicine. These plants have been used to control diabetes in traditional medicinal systems. Objective: In the present study, 50% volume per volume ethanolic extracts of P. lanceolata, A. pseudalhagi, and C. bonduc subjected to in vitro analysis of antidiabetic effect by alpha‑amylase and alpha‑glucosidase inhibitory assay. Materials and Methods: Inhibitory activity of the hydro‑ethanolic extract of the all three plants individually against alpha‑amylase enzyme and alpha‑glucosidase enzyme were examined in different concentrations (3.90–500 µg/mL), where acarbose used as a positive control. Results: The percentage inhibition of P. lanceolata showed the highest alpha‑amylase and alpha‑glucosidase inhibitory activity. Half‑maximal inhibitory concentration value P. lanceolata was found to be 4.83 µg/ml and 11.94 µg/ml for alpha‑amylase and alpha‑glucosidase inhibition. Conclusion: This study suggests that the hydro‑ethanolic extract of all three plants have antidiabetic property, among these three plants P. lanceolata showed potent enzyme inhibition as compared to other plant extracts and standard acarbose. Keywords:Alpha‑amylase, Alpha‑glucosidase, Antidiabetic, Hydro‑ethanolic, in vitro.View:PDF (1.23 MB)