In vitro studies on the effect of 8-(4-chlorophenylthio) cAMP on selected biochemical parameters in Sertoli cells isolated from testis of transgenic mice

Abstract

The central objective of present study was to know the effect of 8-(4-chlorophenylthio) cyclic 3 5 adenosine monophosphate (cpt-cAMP: 5000 ng/ml) on selected biochemical parameters in Sertoli cells isolated from testis of mice over-expressing bovine growth hormone. The results indicated that cpt-cAMP treated Sertoli cells isolated from mice over-expressing bGH showed a significant increase (p<0.001) in the levels of total proteins (33.78%), DNA (167.81%), RNA (152.38%) and lactic acid (49.56%) levels as compared to treated normal Sertoli cells over a period of five days. However, surprisingly, cpt-cAMP-induced effects in Sertoli cells isolated from testis of transgenic and normal mice were significantly lower as compared to their respective controls. From the results, it can be concluded that cpt-cAMP-induced changes in selected biochemical variables might indicate reversal effects against GH-regulation of protein synthesis and lactate production. These results provide basic information related to the response of GH against excess levels of second messengers in the regulation of metabolic fate of Sertoli cells.