Abstract
The aim of the present study was to investigate the changes in selected biochemical variables in Sertoli cells isolated from testis of mice (PEPCK-bGH) overexpressing growth hormone. Significant increase in the levels of total proteins (108.33%; p<0.001), lactic acid (95.23%; p<0.001), DNA (155.42%; p<0.001) and RNA (90.00%; p<0.001) were observed in transgenic Sertoli cells as compared to normal Sertoli cells. Further, cultured transgenic Sertoli cells exhibited distinct nucleus over a period of 5 days and on day 7, exhibited accelerated proliferation as compared to normal Sertoli cells. From the results, it can be concluded that growth hormone at least in part mediates modulation of selected biochemical variables to influence Sertoli cell morphology thereby spermatogenesis in mice.
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