Abstract
1. Pathways of lipogenesis in rabbit liver and adipose tissue were investigated utilizing tissue slice incubations coupled with activities of relevant enzymes. 2. Acetate was the major carbon source for fatty acid synthesis and high rates of lipogenesis were noted for adipose tissue (perirenal and subcutaneous) and liver. 3. The citrate-cleavage pathway and malate-transhydrogenation pathway play only a minor role in fatty acid synthesis in rabbit liver and adipose tissue based on limited glucose incorporation into fatty acids, very low activities of ATP-citrate lyase and NADP-malate dehydrogenase and results from studies using radioactive glutamate. 4. High activities of NADP-isocitrate dehydrogenase and glucose-6-phosphate dehydrogenase were consistent with the isocitrate pathway and the pentose pathway being the major sources of reducing equivalents for lipogenesis in rabbit tissues.
Published Version
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