Abstract
We evaluated the effect of different temperatures and incubation periods on determination of urinary albumin in micro quantities (microalbuminuria). To simulate different physiological and pathological conditions, six human urine samples of albumin concentrations ranging from 2 - 92 micrograms/ml were incubated in vitro at 4, 22, 37, 40, 45 and 56 degrees Centigrade for 4, 10, 24 and 48 hours. The data of the effects of various temperature changes and incubation periods on urine microalbumin concentration fitted a cubic equation of type Y = A+B (T) t+C (T) t 2+D (T) t 3; where A is a constant and B, C and D are temperature dependant constants. Utilizing these equations, the effect of temperature changes between 4 and 56 degrees Centigrade on microalbumin can be predicted. The findings from above experiment suggest that physiological and pathological conditions that cause urine samples to incubate at 4, 22, 37, 40 and 45 degrees Centigrade for 48 hours do not result in significant loss in microalbumin concentrations. However, conditions that cause urine samples to stay at 56 degrees Centigrade for 24 hours or longer would result in a significant loss of microalbumin concentration.
Published Version
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