In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

Abstract

Lysimachia nummularia L. is a perennial medicinal and aromatic herb. In this study, the effects of solid and liquid culture environments on micropropagation of L. nummularia were investigated. Nodes were used as an explant type in the experiments. Different combinations of thidiazuron (TDZ: 0.05-0.80 mg L-1) and indole-3-butyric acid (IBA: 0.10 mg L-1) were used as growth regulators in Murashige and Skoog (MS) basal environment. Agar was put in to solidify the solid nutrient media. No agar was put in the liquid environment. In solid and liquid experiments, the most count of shoots was obtained in MS nutrient environment with 0.40 mg L-1 TDZ + 0.10 mg L-1 IBA. The longest shoot was detected in the nutrient environment including 0.05 mg L-1 TDZ + 0.10 mg L-1 IBA in both culture media. The best results for shoot count and percentage of regeneration were determined in the solid nutrient environment according to in vitro propagation values. On the other hand, the best results for shoot length were found in the liquid culture environment. The use of high TDZ in both culture media negatively affected shoot lengths. The regenerated plants were successfully acclimatized to aquatic conditions. The results of this study may help more efficient propagation of L. nummularia by tissue culture.