Uzun Yapraklı Üçgülün (Trifolium pannonicum ssp. elongatum) Hipokotil ve Yaprak Sapı Eksplantından in vitro Çoğaltılması

Abstract

The study reports effects of various concentrations of Kinetin and α- Naftalin asetik asit (NAA) (kinetin and 0.1+ 0.1, 0.2, 0.4 NAA mg/l) on shoot regeneration (percentage, number and length of shoot) of Trifolium pannonicum ssp. elongatum from hypocotyl and petiole of cotyledon leaf. The maximum (91.7%) shoot regeneration frequency (25%) was recorded on hypocotyl explant on Murashige and Skoog ( MS) medium containing 1 mg/l Kinetin + 0.4 mg/l NAA; whereas, minimum shoot regeneration frequency (25%) was in medium containing 1 mg/l Kinetin + 0.1 NAA respectively, in both hypcotyl and petiole of cotyledon leaf explant. The highest number of 11.2±1.3 shoots per explant was recorded on MS medium containing 1 mg/l Kinetin+0.4 mg/l NAA and the lowest number of 2.5±0.6 was in medium containing 1 mg/l Kinetin+0.1 mg/l NAA respectively. The longest shoots (3.5±0.5 cm) were recorded on MS medium containing 1 mg/l kinetin+ 0,1 mg/L NAA, and the smallest shoots (1.5±0.2 cm) were recorded on MS medium containing 1 mg/l kinetin + 0.4 NAA. Maximum frequency of 95% roots was recorded on MS medium containing 1 mg/l IBA.