In vitro shoot regeneration in Myracrodruon urundeuva Fr. All

Tecla Dos Santos Silva,Priscila Tavares Fonseca,José Raniere Ferreira De Santana,Rosembrando Sosthenes Leite Carvalho Filho

doi:10.1590/1983-40632021v5169269

Abstract

ABSTRACT Myracrodruon urundeuva Fr. All. is a tree threatened with extinction, which has wood and medicinal potential. This study aimed to analyze the in vitro shoot regeneration in M. urundeuva, in order to increase the species multiplication. Two experiments were conducted: 1) concentrations of 6-benzylaminopurine (BAP) (0.0, 2.0, 4.0, 8.0 and 16.0 µM), in association with naphthaleneacetic acid (NAA) (0.0, 1.5 and 3.0 µM), in explants (cotyledon, hypocotyl and cotyledonary node); 2) concentrations of meta-topolin (mT) (0.0, 2.0, 4.0, 8.0, 16.0 and 32.0 µM) in explants (biaxillary, medial uniaxillary and apical basal nodal segment). The percentage of explants responsive to shoot regeneration, percentage of callus explants, number of shoots and shoot length were evaluated. In the first experiment, the shoot regeneration occurred only in explants of the cotyledonary node and hypocotyl type, with the highest responsiveness percentage (76.67 %) and number of shoots (1.97 and 1.63) obtained for the cotyledonary node in the presence of 3.0 µM of NAA in association with 2.0 (1.97 shoots/explant) and 4.0 µM (1.63 shoots/explant) of mT. In the second experiment, the resolution of the obtained quadratic equation indicates that the use of basal explant with 24.59 µM of mT added to the culture medium leads to the highest number of shoots (1.86). However, despite the mT having increased the mean number of shoots, all treatments containing this cytokinin showed callus formation. As a conclusion, it is possible to regenerate shoots in M. urundeuva from the cotyledonary node using BAP in association with NAA.

Highlights

IntroductionIs a tree belonging to the Anacardiaceae family, popularly known in Brazil as ‘aroeira-preta’ or ‘aroeira-dosertão’ (Lima 2011)
Other authors have reported the oxidation of explants of woody species such as blueberry (Vaccinium spp.) (Rosa et al 2009), pomegranate (Punica granatum L.) (Dias et al 2013), rosewood [Dalbergia nigra (Vell.) Allemão ex Benth.] (Sartor et al 2013) and sabiá (Mimosa caesalpiniifolia Benth.) (Bezerra et al 2014)
The triggering of responses for shoot regeneration occurred only in the cotyledonary node and hypocotyl type explants

Summary

Introduction

Is a tree belonging to the Anacardiaceae family, popularly known in Brazil as ‘aroeira-preta’ or ‘aroeira-dosertão’ (Lima 2011). The species is part of the Caatinga biome, which occupies about 11 % of the Brazilian territory and comprises herbaceous, shrub and arboreal plants (Drumond et al 2016). It has a great potential for the recovery of degraded areas and for the composition of agroforestry systems (Maia 2012). Its wood is highly valued in external works and workpieces (Lima 2011).

Objectives

Methods

Results